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KMID : 0380020050200040271
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Korean Journal of Biotechnology and Bioengineering
2005 Volume.20 No. 4 p.271 ~ p.277
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The Effect of Concentration of Glucose and Salts on both the Growth and the Production of Lipid and DHA of Thraustochytrium aureum ATCC 34304
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Kim Won-Ho
Chung Young-Soo
Park Cheon-Eik
Hur Byung-Ki
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Abstract
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The polyunsaturated fatty acids (PUFAs) are mostly synthesized by the marine original microorganisms. In this study, the effect of concentrations of glucose, NaCl, and on both the growth and the production of lipid and Docosahexaenoic acid (DHA) was investigated using Thraustochytrium aureum ATCC 34304. increased according to the increase of initial glucose concentration until 15 g/L of glucose. The maximum values of were 0.18 g/g and 0.41 g/g respectively at 15 g/L of glucose. The biomass yield, , however, was constant as 0.378 g/g regardless of initial glucose concentrations in the range of 5 g/L to 25 g/L. The specific growth rate of T. aureum increased also with initial glucose concentration from 5 g/L and 15 g/L. The maximum value was at 15 g/L of glucose, and the growth rate decreased to and remained constant according to initial sugar concentration in the range of higher than 15 g/L of glucose. The concentration of NaCl was changed from 0 g/L to 48 g/L, in order to study the characteristics of the growth and the production of lipid and DHA according to NaCl concentration. The growth and the production of lipid as well as DHA stopped all at 0 g/L of NaCl and the maximum values of all the three variables occurred at 24 g/L. The effect of concentration was also investigated not to find the growth and the production of lipid and DHA at 0 g/L. However, the growth and the Production with concentration increased to reach the maximum values at 18 g/L of .
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KEYWORD
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Thraustochytrium aureum, docosahexaenoic acid, DHA, Lipid, polyunsaturated fatty acids, PUFAs, specific growth rate
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